金叶败毒颗粒抗炎与免疫药理作用的研究

金叶败毒颗粒(JY)能明显抑制角叉莱胶引起的大鼠足跖肿胀和组织胺引起的大鼠皮肤毛细血管通透性增高,提高小鼠腹腔巨噬细胞吞噬功能、血清溶菌酶含量、溶血素形成以及迟发型超敏反应.     

扶正抗白冲剂对微小残留白血病模型小鼠免疫功能及生存期的影响

目的：研究扶正抗白冲剂（FZKBG）对实验小鼠免疫功能及生存期的影响，探讨FZKBG治疗微小残留白血病（MRL）的机理。方法：接种L7212细胞1×10^6／只，接种后第3天腹腔注射环磷酰胺250mg/kg，造成MRL模型上鼠，分成模型对照组、模型给药组，另设正常对照组、正常给药组，分别观察各组小鼠淋巴细胞亚群（CD3^＋、CD4^、CD8^+）改变，免疫器官病理变化和生存期。结果：与模型组比较，FZKBG能明显提高正常小鼠和MRL模型小鼠CD3^＋、CD4^、CD8^＋的百分比和绝对值（P＜0.05,P<0.01)。且明显延长MRL模型小鼠存活期，延长生命率29.6%-60.4%。结论：FZKBG能明显延长MRL模型小鼠存活期，其机理可能是通过提高MRL模型小鼠的免疫功能，抑制体内白血病细胞而实现的。     

双草退黄冲剂1号治疗急性黄疸型肝炎深度黄疸的临床与实验研究

目的观察双草退黄冲剂1号治疗急性黄疸型肝炎深度黄疸患者的临床疗效并探讨其作用机理。方法对34例急性黄疸型肝炎深度黄疸患者(治疗组)在西医常规治疗的基础上加用口服双草退黄冲剂1号治疗4周，分析其黄疸消退情况及疗效等，与同期西医常规治疗22例(对照组)作比较。并通过动物实验观察其对小鼠D-半乳糖胺急性肝损伤、肝脏超氧化物歧化酶(SOD)及血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)的影响。结果治疗组显效率和有效率分别为73.5%(25/34例)和23.5%(8/34例)，对照组为59.1%(13/22例)和40.9%(9/22例)，两组显效率比较差异有显著性(P＜0.05)；实验研究显示双草退黄冲剂1号大、中剂量组肝损伤轻微，疗效明显优于模型组，且使肝脏SOD活性升高及血清ALT、AST降低(P＜0.05，P＜0.01)。结论双草退黄冲剂1号可加快黄疸消退、改善肝功能及减轻肝损伤，其机理可能与提高SOD活性，清除氧自由基有关。     

扶正缓调法治疗慢性肾功能衰竭60例

目的:观察扶正缓调法治疗慢性肾功能衰竭的疗效。方法:将119例患者随机分为治疗组60例,对照组59例。在常规用药基础上,治疗组加服补肾解毒颗粒10~20 g,日1~2次;病重者,辨证加用肾衰灌肠液Ⅰ、Ⅱ号,高位保留灌肠。对照组加服包醛氧淀粉10 g,日3次;大黄胶囊2粒,日3次。结果:治疗组Scr、Ccr、Hb、24 h尿蛋白含量等指标改善程度及近期、远期疗效均显著优于对照组(P<0.05~0.01)。结论:扶正缓调法可显著改善肾功能,提高生存质量,延缓尿毒症的发生。     

无机陶瓷膜超滤精制连花清瘟颗粒的工艺研究

目的 在无机陶瓷膜精制连花清瘟颗粒过程中,考察不同孔径的膜对该体系的适用性,并进行膜过程优化设计.方法 以连花清瘟水提液为研究对象,测定了3种不同孔径膜及不同操作参数下对膜通量及有效成分含量的变化.结果 50 nm膜的膜通量最大,甘草酸及绿原酸转移率最高,最佳大生产过滤条件为操作压差为0.08～0.12 MPa,过滤温度50～60℃,膜面流速3.0 m/s;当过滤液浓缩到原液体积1/10时,加入相同体积的水,有效成分转移率达80%以上,即可结束过滤.采用强酸、强碱交替清洗,膜通量可恢复99%以上.结论 采用无机陶瓷膜超滤技术精制连花清瘟颗粒是可行的,并为陶瓷膜分离技术应用于其它中药水提液的精制提供了依据.     

脂汰清颗粒对实验性动脉粥样硬化兔主动脉弓超微结构的影响

目的：探讨脂汰清颗粒对实验性动脉粥样硬化兔主动脉弓超微结构的影响。方法：将新西兰兔随机选取10只作空白对照组,喂普通饲料;其余按高脂饲料配方1饲喂6周,制成高脂血症模型,再随机分为模型组、脂汰清治疗组、辛伐他汀对照组、山楂精降脂片对照组。从第7周开始,正常组喂全价营养饲料,余组改喂高脂饲料配方2。同时,脂汰清组及两对照组分别给相应溶化药物,空白组和模型组给等量生理盐水,灌胃,连续11周后检测总胆固醇（CHO）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、载脂蛋白AI（apo AI）、载脂蛋白B（apo B）,扫描电镜下观察主动脉弓组织内膜超微结构的病理变化。结果：脂汰清组可显著降低CHO、LDL-C、ApoB（P〈0.05,P〈0.01）,升高HDL-C（P〈0.05）,抑制、修复血管内皮细胞超微结构损伤。结论：脂汰清颗粒有较好的调节血脂代谢、抑制动脉粥样硬化斑块形成的作用。     

Effect of Yiqi Bushen Granule (益气补肾颗粒) on the Peripheral Natural Killer Cell and γ δ T-cell in the Patients with Minimal Residual Leukemia

Objective: To analyze the changes in peripheral natural killer T-cells (NKT) and gammadelta T-cells (γδT-cell) in patients with minimal residual leukemia (MRL) before and after being treated with Yiqi Bushen Granule (益气补肾颗粒, YBG) in order to determine their significance in prognosis of the disease. Methods: Before and after treatment, the changes in 36 patients (16 males and 20 females) receiving long-term (more than 3 months) YBG therapy were analyzed using multi-parameter flow cytometry, with 34 healthy persons (19 males and 15 females) acting as controls. Results: The absolute value and percentage of NKT cells and γδT-cells were all significantly raised after treatment, for NKT cells, 0.52%±0.39% to 0.83%±0.66% and 7.25±7.77 cell/μL to 12.86±11.99 cell/μL, for γδT-cells, 6.08%±3.03% to 7.24%±2.78% and 83.97±48.09 cell/μL to 110.53±54.12 cell/μL, respectively (P0.05 or P0.01). Conclusion: YBG could regulate the immune function and elevate the amount of NKT cells and γδT-cells, thus to kill or suppress the residual leukemic cell in the body, which might be one of the mechanisms of YBG in prolonging the disease-free survival in MRL patients.     

神怡颗粒的质量标准研究

目的:建立神怡颗粒(大枣、甘草、丹参、地黄,等)的质量标准。方法:采用TLC法对神怡颗粒中大枣、甘草、丹参和地黄进行定性鉴别,采用HPLC法测定制剂中甘草酸和甘草次酸的含量。色谱柱为Agilent/ZORBAX Eclipse×DB-C18(4.6mm×250mm,5μm),以甲醇-2.5乙酸水溶液为流动相作梯度洗脱,流速为1mL/min,检测波长为254nm。结果:在薄层色谱中能检出大枣、甘草、丹参和地黄;甘草酸在1.135～9.08μg、甘草次酸在0.71～5.68μg范围内线性良好,回归系数r分别为0.9993和0.9997。结论:建立的分析方法简便可行,专属性强,可用于神怡颗粒的质量控制。     

Implications of decreased hippocampal neurogenesis in chronic temporal lobe epilepsy

Temporal lobe epilepsy (TLE), characterized by spontaneous recurrent motor seizures (SRMS), learning and memory impairments, and depression, is associated with neurodegeneration, abnormal reorganization of the circuitry, and loss of functional inhibition in the hippocampal and extrahippocampal regions. Over the last decade, abnormal neurogenesis in the dentate gyrus (DG) has emerged as another hallmark of TLE. Increased DG neurogenesis and recruitment of newly born neurons into the epileptogenic hippocampal circuitry is a characteristic phenomenon occurring during the early phase after the initial precipitating injury such as status epilepticus. However, the chronic phase of the disease displays substantially declined DG neurogenesis, which is associated with SRMS, learning and memory impairments, and depression. This review focuses on DG neurogenesis in the chronic phase of TLE and first confers the extent and mechanisms of declined DG neurogenesis in chronic TLE. The available data on production, survival and neuronal fate choice decision of newly born cells, stability of hippocampal stem cell numbers, and changes in the hippocampal microenvironment in chronic TLE are considered. The next section discusses the possible contribution of declined DG neurogenesis to the pathophysiology of chronic TLE, which includes its potential effects on spontaneous recurrent seizures, cognitive dysfunction, and depression. The subsequent section considers strategies that may be useful for augmenting DG neurogenesis in chronic TLE, which encompass stem cell grafting, administration of distinct neurotrophic factors, physical exercise, exposure to enriched environment, and antidepressant therapy. The final section suggests possible ramifications of increasing the DG neurogenesis in chronic epilepsy.     

金龙舒胆冲剂抗炎免疫作用研究

研究金龙舒胆冲剂对炎症、免疫功能及体外细菌生长的影响。结果表明，金龙舒胆冲剂能显著抑制大鼠棉球肉芽肿增生，提高小鼠对碳粒的吞噬活性，促进ＰＨＡ 刺激的小鼠淋巴细胞转化，并具有明显的抑菌效应。提示金龙舒胆冲剂具有抗炎、免疫增强及抑菌作用。